Rice black streaked dwarf virus P7-2 forms a SCF complex through binding to Oryza sativa SKP1-like proteins, and interacts with GID2 involved in the gibberellin pathway.

Rice black streaked dwarf virus P7-2 forms a SCF complex through binding to Oryza sativa SKP1-like proteins, and interacts with GID2 involved in the gibberellin pathway.

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As a core subunit of the SCF complex that promotes protein degradation through the 26S proteasome, S-phase kinase-associated protein 1 (SKP1) plays important roles in multiple cellular processes in eukaryotes, including gibberellin (GA), jasmonate, ethylene, auxin and light responses.P7-2 encoded by Rice black streaked dwarf virus (RBSDV), a devastating viral Browband Headstall pathogen that causes severe symptoms in infected plants, interacts with SKP1 from different plants.However, whether RBSDV P7-2 forms a SCF complex and targets host proteins is poorly understood.In this study, we conducted yeast two-hybrid assays to further explore the interactions between P7-2 and 25 type I Oryza sativa SKP1-like (OSK) proteins, and found that P7-2 interacted with eight OSK members with different binding affinity.Co-immunoprecipitation assay further confirmed the interaction of P7-2 with OSK1, OSK5 and OSK20.

It was also shown that P7-2, together with OSK1 and O.sativa Cullin-1, was able to form the SCF complex.Moreover, yeast two-hybrid assays revealed that P7-2 interacted with gibberellin insensitive dwarf2 (GID2) from rice and maize plants, which is essential for regulating Ski de fond - Homme - Bottes - Back country the GA signaling pathway.It was further demonstrated that the N-terminal region of P7-2 was necessary for the interaction with GID2.Overall, these results indicated that P7-2 functioned as a component of the SCF complex in rice, and interaction of P7-2 with GID2 implied possible roles of the GA signaling pathway during RBSDV infection.